Spatially Resolved Whole Transcriptome Profiling of Fibroblastic Foci in Idiopathic Pulmonary Fibrosis

Robert Pedley, Michael Eyres, Joesph Bell, Nina Vyas, Herve Barjat1, Donna Davies, Mark Jones, Gayle Marshall, Emily Offer

Tissues are not homogenous but are made of multiple discrete compartments. Bulk sequencing methods take a homogenized snapshot, resulting in a huge loss of complexity.

Single-cell sequencing methods allow for the cataloguing of individual components within a tissue. However, it is only with spatial techniques that the relationships between these components can be analysed. GeoMx Digital Spatial Profiling (DSP) enables quantitative, non-destructive, whole transcriptome (WT) profiling in a single FFPE tissue section.