Super resolution microscopy to unravel complex medicine-induced changes in the protein nano environment

Lauren Toms, Duygu Yilmaz, Herve Barjat, Rebecca Kelly, Isobel Holden, Neil Thomas, Isabel Peset-Martin and Matthew Burnham


Super resolution microscopy (Stochastic Optical Reconstruction Microscopy: STORM) enables a resolution of <30nm. This enhancement allows for visualisation and improved understanding of what is happening at the sub-micrometre level. Here, we are using this technique at the forefront of drug discovery to determine the mechanism of action of a  novel complex medicine.

The aim is to:

  1. To determine the internalisation of Affibody Apoferritin conjugate in breast cancer cell lines
  2. To compare HER2 cluster spatial properties following treatment
  3. To examine treatment and receptor densities in different cellular regions

Created in collaboration with the Biodiscovery Institute, School of Chemistry, the University of Nottingham for the ELRIG Discovery Technologies event. This poster won the Early Career Professional Poster prize.

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