Methods to Examine Neuronal Morphology and Function in Human iPSC-derived Disease Models
Eve Corrie, Isabel Peset-Martin, Hervé Barjat and Emma V. Jones
Methods to Examine Neuronal Morphology and Function in Human iPSC-derived Disease Models
The ability to reprogram somatic cells to stem cells has revolutionised the modelling of human diseases in vitro
Induced pluripotent stem cells (iPSCs) can be used to generate multiple cell types found in the central nervous system (CNS), including various types of neurons, astrocytes, and microglia
Mutations can also be introduced to model monogenic diseases, for example using CRISPR-Cas9. This is particularly advantageous as it allows the comparison of molecular and cellular disease phenotypes against the same isogenic control background
We use the techniques outlined in this poster to examine morphological and functional changes that occur due to disease mutations or drug treatments
Here we present these techniques using an iPSC-derived neuron model from bit.bio that has been engineered to have the mutation associated with Huntington’s disease (HD), as an example of a specific disease model
Methods to Examine Neuronal Morphology and Function in Human iPSC-derived Disease Models
